Fixed angle rotors: all open-top, thinwall tubes, requiring multi-piece metallic caps, are to be filled as far as possible and sealed. For thickwall tubes and bottle assemblies, the minimum fill volume is half of its maximum fill volume.
Swinging bucket rotors: all thinwall tubes should be filled to 2 - 3mm from the top.
All OptiSeal TM and Quick-Seal TM tubes are to be filled per instructions in the Rotors and Tubes manuals (LR-IM-, TLR-IM-, and JR-IM-).
It will be important to note that there are exceptions to these guidelines. More detailed information and fill volumes for each tube and bottle type used in a given rotor is found in each rotor operator's manual and in the Beckman Coulter Rotors and Tubes manuals , which are available from Beckman Coulter.