Most leaks are due to improper handling or sealing. Always refer to the Rotors and Tubes manuals before attempting to use the rotor.
1. OptiSeal™ : Make sure the neck area is dry and tubes are correctly filled.
2. Quick-Seal™ : Fill to the base of the neck leaving a small (approx. ¼" diameter) air bubble and seal with the proper Beckman Coulter heat sealer and seal former. For best results, follow the Quick-Seal™ user's booklet directions.
3. Open top tubes: Thinwall tubes must be capped and filled for use in fixed angled rotors (torque the cap to 120 inch-pounds). In swinging bucket rotors the fill level is 2 - 3mm from the top. Thickwall tubes (without caps) in fixed angled rotors need to be filled between half to three-quarters full. In swinging bucket rotors fill to within 2 - 3mm from top.
4. Bottles: Always ensure all sealing surfaces and O-rings are dry. Wet O-rings can act as a pathway for fluids, causing a leak. Be sure all O-rings are present, in good condition, and are dry.