Beckman Coulter JA-14.50 rotor assembly, biosafe (with dual-locking lid) - INSPECTED

The JA14.50 Tapered Rotor Assy, Bio-Contained fixed-angle rotor comes complete with a secure dual-locking lid and is constructed from high-grade aluminum. This rotor is not only lightweight but also versatile, ideal for a variety of applications including pelleting and density gradient separations. It is perfectly suited for the separation of proteins, lysate and tissue homogenates, nucleic acids, cells, viruses, and blood components. Designed to be used with the Avanti J Series centrifuges, it enhances the efficiency and productivity of your laboratory processes.

Our JA-14.50 rotor assembly (DATE OF MFG. 04/21), biosafe (with dual-locking lid), can be quoted alongside our Beckman Coulter Avanti JXN-30 Centrifuge. Contact Service AI for current availability and pricing for the rotor alone or a centrifuge-and-rotor package tailored to your laboratory's workflow.

Salient Features of the JA-14.50 Fixed-Angle Rotor

The JA-14.50 Fixed-Angle Rotor is designed for use with Beckman Coulter Avanti J Series centrifuges. It is suitable for a wide range of applications, including pelleting cells, bacteria, and food products, as well as separating proteins, viruses, and subcellular fractions.

Key Specifications:

• Maximum Speed: 14,000 RPM

• Maximum Relative Centrifugal Force (RCF): 35,000 × g at maximum radius

• Rotor Capacity: 16 tubes, each with a capacity of 50 mL

• Rotor Angle: 35 degrees

• Material: Anodized aluminum rotor body with Buna-N and ethylene propylene rubber O-rings

• Nominal Rotor Capacity: 800 mL

Design and Features:

• Dual-Locking and Single-Locking Lid Options: The rotor is available with either a dual-locking or single-locking lid for secure operation. The dual-locking lid allows for safe tube handling under a safety hood.

• Fluid-Containment Annulus: The patented design helps retain liquid that may escape from overfilled or leaking tubes, minimizing the risk of contamination.

• Versatile Applications: Ideal for pelleting, phase separation, and binding studies. The rotor can also be used for high-speed centrifugation of biological samples.

Maintenance and Care:

• Lubrication: Regularly apply Spinkote lubricant to metal threads and silicone vacuum grease to O-rings to maintain sealing integrity.

• Cleaning: Wash the rotor with a mild detergent such as Solution 555, rinse with distilled water, and air-dry. Avoid using acetone or harsh chemicals.

• Decontamination: Use RadCon Surface Spray, IsoClean Solution, or Radiacwash for decontamination, following proper safety protocols.

Part Numbers for the JA-14.50 Rotor Assembly

1. Rotor Assembly:

   • JA-14.50 Rotor Assembly with dual-locking lid: B88554

   • JA-14.50 Rotor Assembly with single-locking lid: B88555

2. Lid and O-rings:

   • Dual-locking lid: B19947

   • Single-locking lid: B19952

   • Large O-ring (Buna-N rubber): 885501

   • Small O-ring (ethylene propylene rubber): 961696

3. Lubrication and Cleaning Supplies:

   • Spinkote Lubricant (2 oz): 306812

   • Silicone Vacuum Grease (1 oz): 335148

   • Solution 555 (1 qt): 339555

   • Rotor Cleaning Kit: 339558

Source:

• Title: JA-14.50 Fixed-Angle Rotor Manual

• Publisher: Beckman Coulter, Inc.

• Publication Date: May 2023

• Document ID: B32164AE

This manual provides comprehensive safety, operation, and maintenance instructions for the JA-14.50 Fixed-Angle Rotor, ensuring reliable and safe operation in Beckman Coulter centrifuges.

JA-14.50 Applications: LabPundit Reading

With sixteen 50 mL positions and an 800 mL nominal capacity, the JA-14.50 provides a configuration for laboratories processing multiple samples within established pelleting and separation protocols. LabPundit's Avanti JXN-30 and JA-14.50 application overview discusses cell pelleting, clarification of suspensions, and related workflow considerations.

Rotor Selection and Labware Configuration

Separation objectives, required RCF, sample volume, and tube or adapter configuration together determine the appropriate operating conditions. The JA-14.50 manual provides the applicable labware combinations and operating limits, including adjustments for sample density. For broader context on rotor geometry and configuration choices, see A Startup Guide to Centrifuge Rotors.

Rotor Care and Lifecycle Planning

Cleaning methods, inspection criteria, and service-life requirements are specific to the rotor design. The assembly's manufacture date is one part of its history; condition and manufacturer-defined service limits remain relevant to lifecycle planning. LabPundit's Complete Guide to Centrifuge Rotor Care, Inspection, and Retirement provides context on storage, corrosion, inspection, and retirement considerations. Evidence of cracking, pitting, or other damage warrants withdrawal from service pending a qualified assessment.

Centrifugation Reference Resources

Our Centrifuge FAQ: Essential Information for Users brings together reference information on RPM and RCF, tube fill volumes, consumable reuse, and rotor identification. These articles complement the current rotor, centrifuge, and labware manuals, which remain the primary references for operating procedures.