Why do centrifuge tubes leak?
Service AI
Most leaks are due to improper handling or sealing. Always refer to the Rotors and Tubes manuals before attempting to use the rotor.
1. OptiSeal™ : Make sure the neck area is dry and tubes are correctly filled.
2. Quick-Seal™ : Fill to the base of the neck leaving a small (approx. ¼" diameter) air bubble and seal with the proper Beckman Coulter heat sealer and seal former. For best results, follow the Quick-Seal™ user's booklet directions.
3. Open top tubes: Thinwall tubes must be capped and filled for use in fixed angled rotors (torque the cap to 120 inch-pounds). In swinging bucket rotors the fill level is 2 - 3mm from the top. Thickwall tubes (without caps) in fixed angled rotors need to be filled between half to three-quarters full. In swinging bucket rotors fill to within 2 - 3mm from top.
4. Bottles: Always ensure all sealing surfaces and O-rings are dry. Wet O-rings can act as a pathway for fluids, causing a leak. Be sure all O-rings are present, in good condition, and are dry.
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