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Service AI support guide for centrifuge questions. Please feel free to email us at support@serviceai.us
Filtering by Tag: Ultracentrifuge
Centrifugation is a fundamental technique in biological and bioindustrial research, and the choice of labware—tubes and bottles—plays a crucial role in the efficiency and success of this process. This guide covers key aspects of centrifugation labware, including material selection, design considerations, chemical resistance, and maintenance.
Read MoreCentrifugation is a technique used to separate particles in a solution by applying a centrifugal force. In biological research, these particles often include cell organelles or large molecules.
Read MoreIf your Beckman Coulter Optima ultracentrifuge flags d63; it is an indication that the drive thermostat mounted on the drive has overheated (causing an open circuit) during normal run, or that it did not cool down and get deactivated after a 5 minute waiting period on power up. When a power fail diagnostic occurs during a normal run, the ultracentrifuge will shut down with brake.
If this fault occurs, a Beckman trained and experienced expert will make it a point to check the drive, drive oil, drive fans, and the drive control system of the ultracentrifuge.
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Important Note: To ensure proper electrical installation and checkup as outlined below, it is imperative to engage a certified electrician for power requirements. Furthermore, we highly recommend that a qualified engineer handles the installation and thorough inspection of your Beckman Coulter Optima L ultracentrifuge, while also providing training to your staff on its correct usage and handling of the ultracentrifuge and rotors before operation.
a. Check for correct wall receptacle and voltage at the outlet. Power required is 180 to 264 Volts AC measured as shown. It is advisable to test the line voltage at the plug while the instrument is accelerating and cooling; therefore, we would strictly recommend that the ultracentrifuge always be installed by a Beckman trained engineer. Service AI has Beckman Coulter trained service experts. You can email us at support@serviceai.us, or call us at our technical support line at +1-510-899-1340 for more information.
b. Remove console cover, front panel and top cover.
c. Make sure jumper connector on the power supply boards plugged into the correct voltage range socket J52 A, B, or C. (Select the jumper for the lowest voltage reading during maximum power consumption.)
J52 VOLTAGE RANGE
A 180 - 206
B 207 - 233
C 234- 264
a. Position ultracentrifuge in desired location. Maintain 6" minimum clearance at rear and 3" minimum clearance at each side.
b. Position the four pads provided under the leveling legs. Mark the position of each pad on the floor.
c. Roll out instrument away from pads.
d. Glue (epoxy) pads to floor.
e. Roll instrument back into position and screw the four leveling legs down into the pads until they have bottomed out.
f. Now screw each leveling leg down five additional turns. Make sure all four casters are off the floor.
NOTE: A solenoid inter-lock prevents the chamber door from being opened during operation and/or with the power off. The chamber door can be opened with the door handle only when the power to the ultracentrifuge is on and the vacuum is off.
g. Open the chamber door (see note above). Level the ultracentrifuge as if it had only three legs. Adjust level by placing the spirit level on the drive shaft (crown) and adjust the leveling legs so the air bubble is centered. First level it front- to- rear by adjusting the left front and left rear legs. Then level it left to right by adjusting the right front leg only. (Make sure you do not tilt the instrument). Screw in the right rear leg until it just touches the pad. Now screw it down 1/4 turn more. This does not affect the level of the instrument.
h. After instrument is leveled, make sure all four casters are off the floor and all four leveling legs are still bottomed out.
website: www.serviceai.us; email: support@serviceai.us; technical support: +1-510-899-1340
Most leaks are due to improper handling or sealing. Always refer to the Rotors and Tubes manuals before attempting to use the rotor.
1. OptiSeal™ : Make sure the neck area is dry and tubes are correctly filled.
2. Quick-Seal™ : Fill to the base of the neck leaving a small (approx. ¼" diameter) air bubble and seal with the proper Beckman Coulter heat sealer and seal former. For best results, follow the Quick-Seal™ user's booklet directions.
3. Open top tubes: Thinwall tubes must be capped and filled for use in fixed angled rotors (torque the cap to 120 inch-pounds). In swinging bucket rotors the fill level is 2 - 3mm from the top. Thickwall tubes (without caps) in fixed angled rotors need to be filled between half to three-quarters full. In swinging bucket rotors fill to within 2 - 3mm from top.
4. Bottles: Always ensure all sealing surfaces and O-rings are dry. Wet O-rings can act as a pathway for fluids, causing a leak. Be sure all O-rings are present, in good condition, and are dry.
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The RPM/RCF information is included in each rotor operator's manual. You can also use the G-Calculations program found at this Beckman Coulter website link to obtain this information. Also, many Beckman Coulter centrifuge models can be programmed using RCF ("g") or RPM so that this calculation is not necessary when using these models.
website: www.serviceai.us ; email: support@serviceai.us
To remove a tube when using swinging buckets, remove any tube cap or closure and tube contents. Always invert the buckets when putting them into an autoclave. Autoclave the rotor at 250 degrees Fahrenheit, for 15 minutes at fast exhaust. The tubes or bottles should loosen or soften enough to slide out.
If it is a fixed angle rotor - uncap, vent, and empty the tube; invert the rotor over a clean wash basin, if necessary, with the lid off, and put the rotor into an autoclave. Use the same autoclave cycle as with swinging bucket rotors.
Important Note: You should NEVER try to remove a tube with a sharp object. If a spacer or cap is stuck, DO NOT autoclave; try applying dry ice to the cap or spacer.
Service AI support guide for centrifuge questions. Please feel free to email us at support@serviceai.us
Read MoreFixed angle rotors: all open-top, thinwall tubes, requiring multi-piece metallic caps, are to be filled as far as possible and sealed. For thickwall tubes and bottle assemblies, the minimum fill volume is half of its maximum fill volume.
Swinging bucket rotors: all thinwall tubes should be filled to 2 - 3mm from the top.
All OptiSeal TM and Quick-Seal TM tubes are to be filled per instructions in the Rotors and Tubes manuals (LR-IM-, TLR-IM-, and JR-IM-).
It will be important to note that there are exceptions to these guidelines. More detailed information and fill volumes for each tube and bottle type used in a given rotor is found in each rotor operator's manual and in the Beckman Coulter Rotors and Tubes manuals , which are available from Beckman Coulter.
website: www.serviceai.us ; email: support@serviceai.us
