Introduction to Centrifugation
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Centrifugation is a technique used to separate particles in a solution by applying a centrifugal force. In biological research, these particles often include cell organelles or large molecules.
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Guide to Centrifuge Questions
Service AI support guide for centrifuge questions. Please feel free to email us at support@serviceai.us
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Determining RPM for Desired 'g' Force with a Beckman Coulter Rotor
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The RPM/RCF information is included in each rotor operator's manual. You can also use the G-Calculations program found at this Beckman Coulter website link to obtain this information. Also, many Beckman Coulter centrifuge models can be programmed using RCF ("g") or RPM so that this calculation is not necessary when using these models.
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How to get a stuck tube out of the centrifuge rotor...
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To remove a tube when using swinging buckets, remove any tube cap or closure and tube contents. Always invert the buckets when putting them into an autoclave. Autoclave the rotor at 250 degrees Fahrenheit, for 15 minutes at fast exhaust. The tubes or bottles should loosen or soften enough to slide out.
If it is a fixed angle rotor - uncap, vent, and empty the tube; invert the rotor over a clean wash basin, if necessary, with the lid off, and put the rotor into an autoclave. Use the same autoclave cycle as with swinging bucket rotors.
Important Note: You should NEVER try to remove a tube with a sharp object. If a spacer or cap is stuck, DO NOT autoclave; try applying dry ice to the cap or spacer.
Service AI support guide for centrifuge questions. Please feel free to email us at support@serviceai.us
Read MoreMinimum and Maximum Fill Volumes for Beckman Tubes
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Fixed angle rotors: all open-top, thinwall tubes, requiring multi-piece metallic caps, are to be filled as far as possible and sealed. For thickwall tubes and bottle assemblies, the minimum fill volume is half of its maximum fill volume.
Swinging bucket rotors: all thinwall tubes should be filled to 2 - 3mm from the top.
All OptiSeal TM and Quick-Seal TM tubes are to be filled per instructions in the Rotors and Tubes manuals (LR-IM-, TLR-IM-, and JR-IM-).
It will be important to note that there are exceptions to these guidelines. More detailed information and fill volumes for each tube and bottle type used in a given rotor is found in each rotor operator's manual and in the Beckman Coulter Rotors and Tubes manuals , which are available from Beckman Coulter.
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