Centrifuge FAQ: Essential Information for Users

1. What RPM should I use with my Beckman rotor to achieve the desired "g" force?

The RPM/RCF information is included in each rotor operator's manual. Additionally, Beckman Coulter offers a G-Calculations program on their website to help you determine the correct RPM. Many Beckman Coulter centrifuge models, such as the Avanti J series, can be programmed using either RCF ("g") or RPM, eliminating the need for manual calculations.

2. What should I do if there is a power outage and I can’t open the centrifuge to retrieve my samples?

Refer to the emergency access instructions in your centrifuge operator's manual. Always remember to unplug the centrifuge before attempting to retrieve your samples.

3. How do I remove a stuck tube from my rotor?

For fixed-angle rotors, uncap and empty the tube, then invert the rotor and autoclave it at 121°C for 15 minutes. For swinging bucket rotors, remove any tube cap and contents, invert the buckets, and follow the same autoclave procedure. Avoid using sharp objects to remove tubes. If a spacer or cap is stuck, apply dry ice to facilitate removal.

4. What is the minimum/maximum fill volume for my tubes?

For fixed-angle rotors, open-top thinwall tubes should be filled as much as possible and sealed. Thickwall tubes and bottle assemblies should be filled to at least half of their capacity. Swinging bucket rotors require thinwall tubes to be filled 2-3 mm from the top. Specific instructions for each tube and rotor can be found in the rotor operator's manual.

5. How do I sterilize my tubes?

Sterilization instructions are provided in the Use and Care of Centrifuge Tubes and Bottles (publication IN-192), which is included with each purchase. Autoclaving can reduce the lifespan of some materials, so cold sterilization methods are recommended when possible.

6. Can I autoclave my rotor, adapters, or tools?

Most metal rotors can be autoclaved at 121°C for up to an hour. Specific sterilization and decontamination instructions are available in the rotor's operator manual.

7. What materials are Beckman rotors, tubes, and adapters made of?

Rotors: Typically made of titanium or aluminum, with some components made of epoxy resin or stainless steel.

Tubes: Available in a variety of materials, including polyallomer, polypropylene, polycarbonate, and more. Detailed material information can be found in the rotor operator's manual.

8. I am getting a "DISK" error message on my ultracentrifuge. What does it mean?

The "DISK" error indicates that your rotor has a missing or damaged overspeed disk. Replace the disk using a new one provided with the rotor and allow the adhesive to cure for 2 to 24 hours before using the rotor again. Detailed replacement instructions are in the Rotors and Tubes manual.

9. Are Beckman bottles and tubes reusable?

Most bottles are reusable but require inspection between runs. However, OptiSeal™, Quick-Seal™, and open-top tubes are designed for single use only. Refer to the Use and Care of Centrifuge Tubes and Bottles manual for more information.

10. Can I purchase sterile tubes from Beckman?

Beckman does not sell pre-sterilized tubes, but they can be sterilized using autoclaving or cold sterilization methods. Be aware that autoclaving may reduce the life of the tubes. Refer to Use and Care of Centrifuge Tubes and Bottles (publication IN-192)

11. How can I remove a stripped plug from my vertical tube rotor?

Insert a flat-head screwdriver into the middle of the plug and use pliers or vise grips to turn the screwdriver. Replace the damaged plug with a new one, ensuring that Spinkote is applied to the threads.

12. Why do my centrifuge tubes leak?

Leaks often result from improper handling or sealing. Ensure that the tubes are correctly filled, sealed, and that all O-rings are dry and in good condition. Specific guidelines for different tube types can be found in the Rotors and Tubes manuals.

13. Can I spin hazardous solutions in my centrifuge?

Flammable materials like explosives, ethyl alcohol, chloroform, or toluene should not be run in a Beckman centrifuge. Always refer to the Centrifuge Safety booklet (publication IN-194) and the specific centrifuge instruction manual for safe handling practices.

14. Where can I find the serial number on my Beckman centrifuge and rotor?

Centrifuges: The serial number tag is located near the power cord on the back of the centrifuge.

Rotors: The serial number is typically engraved on the top, on the rotor lids, or sometimes on the bottom of the rotor.

This FAQ provides critical information to ensure safe and effective centrifuge operation. For further details, always refer to the specific rotor and centrifuge operator manuals.

Elevate Your Research with the Beckman Coulter Optima XPN-80 and SW 41 Ti Rotor

In the fast-paced world of scientific research, the precision and reliability of your equipment can make or break the success of your experiments. The Beckman Coulter Optima XPN-80 Ultracentrifuge, paired with the SW 41 Ti rotor, offers an unmatched combination of speed, precision, and versatility, making it a must-have in any serious research laboratory.

Advanced Separation Capabilities: The Optima XPN-80 Ultracentrifuge is engineered for ultra-high-speed operations, reaching up to 80,000 rpm. This capability allows for the efficient separation of biological macromolecules, including nucleic acids, proteins, and viruses. When paired with the SW 41 Ti swinging-bucket rotor, this ultracentrifuge excels in running density gradient centrifugation—a critical process in isolating and purifying delicate biomolecules.

Key Applications:

• Virology Research: Rapidly pellet virus particles for further analysis.

• Cell Biology: Isolate cellular organelles like mitochondria and ribosomes with high yield and purity.

• Biochemistry: Perform complex biochemical analyses involving proteins, lipoproteins, and other macromolecules.

• Genetic Studies: Extract high-quality nucleic acids which are pivotal for genetic research and diagnostics.

The Optima XPN-80’s advanced software interface provides unprecedented control over centrifugation parameters, allowing researchers to fine-tune their processes with ease. Customizable run profiles, detailed temperature control, and real-time performance monitoring ensure that even the most sensitive samples are handled with care.

Designed with the user's safety in mind, the Optima XPN-80 features comprehensive safety locks, imbalance detection, and over-speed protection. This attention to detail guarantees not only the safety of the laboratory personnel but also the integrity of the samples being processed.

Why Choose the Optima XPN-80 with SW 41 Ti Rotor?

• Enhanced Productivity: Process multiple samples simultaneously with confidence, knowing each run delivers consistent and reproducible results.

• Reliability: Beckman Coulter’s commitment to quality means you can rely on this ultracentrifuge to perform day in and day out without fail.

• Investment in Future: Equip your lab with technology that stays relevant through years of research developments and laboratory demands.

The Beckman Coulter Optima XPN-80 Ultracentrifuge and SW 41 Ti rotor combination is not just an investment in a piece of equipment—it's an investment in the future of your research. Whether you're at the forefront of vaccine development, pioneering new genetic research, or exploring the complexities of cellular structures, this ultracentrifuge will be a pivotal part of your success.

Discover how the Optima XPN-80 can transform your research capabilities. Visit us at ServiceAI.US to learn more and arrange a demo today. Let us help you advance the boundaries of what's possible in your laboratory.

Service AI is pleased to announce that we currently have the Beckman Optima XPN-80 available for sale in our inventory. To inquire about the system, please do not hesitate to contact us via phone at 510-899-1340 or email us at support@serviceai.us.