Centrifuge FAQ: Essential Information for Users

1. What RPM should I use with my Beckman rotor to achieve the desired "g" force?

The RPM/RCF information is included in each rotor operator's manual. Additionally, Beckman Coulter offers a G-Calculations program on their website to help you determine the correct RPM. Many Beckman Coulter centrifuge models, such as the Avanti J series, can be programmed using either RCF ("g") or RPM, eliminating the need for manual calculations.

2. What should I do if there is a power outage and I can’t open the centrifuge to retrieve my samples?

Refer to the emergency access instructions in your centrifuge operator's manual. Always remember to unplug the centrifuge before attempting to retrieve your samples.

3. How do I remove a stuck tube from my rotor?

For fixed-angle rotors, uncap and empty the tube, then invert the rotor and autoclave it at 121°C for 15 minutes. For swinging bucket rotors, remove any tube cap and contents, invert the buckets, and follow the same autoclave procedure. Avoid using sharp objects to remove tubes. If a spacer or cap is stuck, apply dry ice to facilitate removal.

4. What is the minimum/maximum fill volume for my tubes?

For fixed-angle rotors, open-top thinwall tubes should be filled as much as possible and sealed. Thickwall tubes and bottle assemblies should be filled to at least half of their capacity. Swinging bucket rotors require thinwall tubes to be filled 2-3 mm from the top. Specific instructions for each tube and rotor can be found in the rotor operator's manual.

5. How do I sterilize my tubes?

Sterilization instructions are provided in the Use and Care of Centrifuge Tubes and Bottles (publication IN-192), which is included with each purchase. Autoclaving can reduce the lifespan of some materials, so cold sterilization methods are recommended when possible.

6. Can I autoclave my rotor, adapters, or tools?

Most metal rotors can be autoclaved at 121°C for up to an hour. Specific sterilization and decontamination instructions are available in the rotor's operator manual.

7. What materials are Beckman rotors, tubes, and adapters made of?

Rotors: Typically made of titanium or aluminum, with some components made of epoxy resin or stainless steel.

Tubes: Available in a variety of materials, including polyallomer, polypropylene, polycarbonate, and more. Detailed material information can be found in the rotor operator's manual.

8. I am getting a "DISK" error message on my ultracentrifuge. What does it mean?

The "DISK" error indicates that your rotor has a missing or damaged overspeed disk. Replace the disk using a new one provided with the rotor and allow the adhesive to cure for 2 to 24 hours before using the rotor again. Detailed replacement instructions are in the Rotors and Tubes manual.

9. Are Beckman bottles and tubes reusable?

Most bottles are reusable but require inspection between runs. However, OptiSeal™, Quick-Seal™, and open-top tubes are designed for single use only. Refer to the Use and Care of Centrifuge Tubes and Bottles manual for more information.

10. Can I purchase sterile tubes from Beckman?

Beckman does not sell pre-sterilized tubes, but they can be sterilized using autoclaving or cold sterilization methods. Be aware that autoclaving may reduce the life of the tubes. Refer to Use and Care of Centrifuge Tubes and Bottles (publication IN-192)

11. How can I remove a stripped plug from my vertical tube rotor?

Insert a flat-head screwdriver into the middle of the plug and use pliers or vise grips to turn the screwdriver. Replace the damaged plug with a new one, ensuring that Spinkote is applied to the threads.

12. Why do my centrifuge tubes leak?

Leaks often result from improper handling or sealing. Ensure that the tubes are correctly filled, sealed, and that all O-rings are dry and in good condition. Specific guidelines for different tube types can be found in the Rotors and Tubes manuals.

13. Can I spin hazardous solutions in my centrifuge?

Flammable materials like explosives, ethyl alcohol, chloroform, or toluene should not be run in a Beckman centrifuge. Always refer to the Centrifuge Safety booklet (publication IN-194) and the specific centrifuge instruction manual for safe handling practices.

14. Where can I find the serial number on my Beckman centrifuge and rotor?

Centrifuges: The serial number tag is located near the power cord on the back of the centrifuge.

Rotors: The serial number is typically engraved on the top, on the rotor lids, or sometimes on the bottom of the rotor.

This FAQ provides critical information to ensure safe and effective centrifuge operation. For further details, always refer to the specific rotor and centrifuge operator manuals.